Immunoblot
Lane 1 and 2: HepG2 cell lysateLane 3 and 4: Hep3B cell lysateLane 5 and 6: K562 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Secondary Antibody Dilution: 1:1000Gene Name: TFAMSubmitted by: AnonymousWestern blot
TFAM antibody - N-terminal region validated by WB using bEND3 cell lysate at lane1:10 ug , lane2:20 ug.| Product Name | Rabbit anti-TFAM polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCF6, MtTF1, mtTFA, TCF6L1, TCF6L2, TCF6L3 |
| Immunogen | The immunogen for anti-TFAM antibody: synthetic peptide directed towards the N terminal of human TFAM |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFAM |
|---|---|
| Gene Synonyms | TCF6 MtTF1 mtTFA TCF6L1 TCF6L2 TCF6L3 |
| Gene Full Name | Transcription factor A, mitochondrial |
| Gene Summary | The function remains unknown.This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells. |
| Alternative Names | TCF6 MtTF1 mtTFA TCF6L1 TCF6L2 TCF6L3 |
| Molecular Weight(MW) | 29kDa |
| Sequence | 246 amino acids |
Immunoblot
Lane 1 and 2: HepG2 cell lysateLane 3 and 4: Hep3B cell lysateLane 5 and 6: K562 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Secondary Antibody Dilution: 1:1000Gene Name: TFAMSubmitted by: Anonymous
Western blot
TFAM antibody - N-terminal region validated by WB using bEND3 cell lysate at lane1:10 ug , lane2:20 ug.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM105326