NEK1-related kinase 1) Antibody

Catalog Number：OM113384

Reactivity: Human

Applaction: WB

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Catalog Number: OM113384

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OM113384-100ul

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50ul

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20ul

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Product Profile

Product Name NEK1-related kinase 1) Antibody

Antibody Type Primary Antibodies

Immunogen Synthetic peptide directed towards the middle region of human NEK1

Key Feature

Clonality Polyclonal

Isotype IgG

Host Species Rabbit

Tested Applications WB

Species Reactivity Human

Concentration 1mg/ml

Purification Affinity purified

Target Information

Gene Symbol NEK1

Gene Synonyms PCGF3

Gene Full Name NIMA (never in mitosis gene a)-related kinase 1

Gene Summary NEK1 phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. NEK1 is implicated in the control of meiosis.

Alternative Names RNF3
DONG1
RNF3A

Molecular Weight(MW) 143kDa

Sequence 1258 amino acids

Database Links

Entrez Gene 4750

SwissProt ID Q96PY6

Protein Accession NP_036356

Application

Application

Host: Rabbit
Target Name: PCGF3
Sample Type: Fetal Muscle lysates
Antibody Dilution: 1.0ug/ml

Application
WB Suggested Anti-PCGF3 Antibody Titration: 0.125ug/ml
ELISA Titer: 1:12500
Positive Control: Raji cell lysate
PCGF3 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells

Application

Host: Rabbit
Target Name: F13B
Sample Tissue: Human DLD1
Antibody Dilution: 1.0ug/ml

Application
Rabbit Anti-F13B antibody
Catalog Number: ARP41999
Formalin Fixed Paraffin Embedded Tissue: Human Fetal Liver
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec

Application
Rabbit Anti-F13B antibody
Catalog Number: ARP41999
Formalin Fixed Paraffin Embedded Tissue: Human Vessel
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec

Application
WB Suggested Anti-FBXO25 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human heart

Application

Host: Rabbit
Target Name: MARCH5
Sample Type: U937 Whole Cell lysates
Antibody Dilution: 1.0ug/ml

Application
WB Suggested Anti-SLC8A3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 721_B cell lysate

Application
WB Suggested Anti-SULT6B1 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate

Application
WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human heart

Application
WB Suggested Anti-ZNF599 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 293T cell lysate

Application
WB Suggested Anti-SSX4B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human kidney

Application
WB Suggested Anti-UBQLN1 Antibody Titration: 1 ug/ml
Positive Control: 293T cells lysate

Application
Immunohistochemistry with Human Lung, respiratory epethelium tissue at an antibody concentration of 5.0ug/ml using anti-UBQLN1 antibody (ARP54958_P050).

Application
Anti-UBQLN1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. UBQLN1 Antibody ARP54958_P050 concentration 5 ug/ml.

Application
WB Suggested Anti-Vwa5a Antibody
Titration: 1.0 ug/ml
Positive Control: Mouse Kidney

Application

Host: Rabbit
Target Name: SPATA2
Sample Type: HCT15 Whole Cell lysates
Antibody Dilution: 1.0ug/ml

Application
WB Suggested Anti-ADAM8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: PANC1 cell lysate

Application
WB Suggested Anti-ORMDL3 Antibody
Titration: 1.0 ug/ml
Positive Control: Jurkat Whole Cell

Application

Host: Rabbit
Target Name: NR4A1
Sample Tissue: Human Hela
Antibody Dilution: 1.0ug/ml

Application
Rabbit Anti-NR4A1 Antibody
Catalog Number: ARP31941
Paraffin Embedded Tissue: Human Stomach
Cellular Data: Mucous Cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Application
Rabbit Anti-NR4A1 Antibody
Catalog Number: ARP31941
Paraffin Embedded Tissue: Human Liver
Cellular Data: Hepatocytes
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Application
Lanes:
Lane 1: 50ug mouse embryonic fibroblast lysate
Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate
Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Donkey anti-Rabbit-HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
PTRF
Submitted by:
Libin Liu, Boston University.

Application

Host: Rabbit
Target Name: PTRF
Sample Tissue: Mouse Lung
Antibody Dilution: 1.0ug/ml

Application
IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.

Application
Lanes:
Lane 1: 5 ug 293T cell lysate
Lane 2: 10 ug 293T cell lysate
Lane 3: 20 ug 293T cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
HMGA2
Submitted by:
Anonymous
HMGA2 is supported by BioGPS gene expression data to be expressed in HEK293T

Application
WB Suggested Anti-TRIM32 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate

Application
PKLR antibody - N-terminal region (ARP41699_T100) validated by WB using HepG2 cell lysate at 1.0ug/ml.
There is BioGPS gene expression data showing that PKLR is expressed in HepG2

Application
Rabbit Anti-PKLR Antibody
Catalog Number: ARP41699
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Application

Sample Type: Equine Cartilage Explants
Primary Dilution: 1:800
Secondary Antibody: Bio-Rad 170-5046
Secondary Dilution::100,000
Image Submitted By: Adam Williams
University of Nottingham

Application
WB Suggested Anti-MMP1 Antibody
Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate
Primary Antibody Dilution : 1:500
Secondary Antibody : Anti-rabbit-HRP
Secondry Antibody Dilution : 1:2000
Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland Baltimore

Application

Host: Rabbit
Target Name: MMP1
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml

Application

Host: Rabbit
Target Name: MARCH5
Sample Type: Human Fetal Kidney
Antibody Dilution: 1.0ug/ml

Application

Host: Rabbit
Target Name: G6pc
Sample Tissue: Mouse Lung
Antibody Dilution: 1.0ug/ml

Application

Host: Rabbit
Target Name: G6PC
Sample Tissue: Mouse Testis
Antibody Dilution: 1ug/ml

Application

Host: Rabbit
Target Name: SPPL2B
Sample Type: 293T Whole cell lysates
Antibody Dilution: 1.0ug/ml

Application
WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Spleen

Application
WB Suggested Anti-NUP35 Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysate

Application
WB Suggested Anti-Amigo2 Antibody
Titration: 1.0 ug/ml
Positive Control: Rat Heart

Application
WB Suggested Anti-ACO2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysate
ACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

Application
ACO2 antibody - N-terminal region (ARP56301_P050) validated by WB using Proximal kidney tubules purfied from cortex at 5.0ug/ml.

Application
WB Suggested Anti-FFAR1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Liver

Application
WB Suggested Anti-GLIS2 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:12500
Positive Control: HepG2 cell lysate

Application
WB Suggested Anti-RBM14 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: SP2/0 cell lysate

Application
Lanes:
Lane 1: 50ug Hela Lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
RBM14
Submitted by:
Archa Fox, University of Western Australia

Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid

Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles

Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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Data sheet for OM113384

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Product Name	NEK1-related kinase 1) Antibody
Antibody Type	Primary Antibodies
Immunogen	Synthetic peptide directed towards the middle region of human NEK1

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WB
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	NEK1
Gene Synonyms	PCGF3
Gene Full Name	NIMA (never in mitosis gene a)-related kinase 1
Gene Summary	NEK1 phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. NEK1 is implicated in the control of meiosis.
Alternative Names	RNF3 DONG1 RNF3A
Molecular Weight(MW)	143kDa
Sequence	1258 amino acids

Entrez Gene	4750
SwissProt ID	Q96PY6
Protein Accession	NP_036356

Form	Liquid
Storage Instructions	Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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