Application
Anti-CD5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CD5Application
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.| Product Name | CD5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4H3] to CD5May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2. |
| Immunogen | Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4H3 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCWB |
| IHC 1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | T1 LEU1 CD5 |
|---|---|
| Target | CD5 |
| Tissue Specificity | is specific for human CD5 (NP_055022). |
Application
Anti-CD5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CD5
Application
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.| Application Notes | IHC 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Data sheet for OM245582