Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody.Application
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.| Product Name | CD5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6F7] to CD5May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2. |
| Immunogen | Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6F7 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| Flow Cytometry 1:100: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | T1 LEU1 CD5 |
|---|---|
| Target | CD5 |
| Tissue Specificity | is specific for human CD5 (NP_055022). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody.
Application
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.| Application Notes | Flow Cytometry 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Data sheet for OM245585