Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).Application
Western blot analysis of ALPP using rabbit polyclonal ALPP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPP gene.Application
ALPP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ALPP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP |
| Product description | There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.1) Stec, B., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010) : |
| Immunogen | This ALPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the N-terminal region of human ALPP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALPP |
|---|---|
| Alternative Names | Alkaline phosphatase placental type Alkaline phosphatase Regan isozyme Placental alkaline phosphatase 1 PLAP-1 ALPP PLAP |
| Molecular Weight(MW) | 58 kDa |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application
Western blot analysis of ALPP using rabbit polyclonal ALPP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPP gene.
Application
ALPP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM270952