Application
Immunohistochemistry of CCR5 in human lymph node tissue with CCR5 antibody at 20 μg/mL.Application
Immunohistochemistry of CCR5 in human lymph node tissue with CCR5 antibody at 2.5 μg/ml.Application
Western blot analysis of CCR5 in THP-1 whole cell lysate with CCR5 antibody at 1 μg/mL.| Product Name | CCR5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCR5 Antibody: CKR5, CCR-5, CD195, CKR-5, CCCKR5, CMKBR5, IDDM22, CC-CKR-5, C-C chemokine receptor type 5, CHEMR13, C-C CKR-5 |
| Product description | CCR5 Antibody: Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.1) Dimitrov, DS. Cell 1997; 91:721-30 |
| Immunogen | CCR5 antibody was raised against a 15 amino acid peptide near the amino terminus of human CCR5. The immunogen is located within the first 50 amino acids of CCR5. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| CCR5 antibody can be used for Western blot at 1 μg/mL. CCR5 antibody can also detect CCR5 by immunohistochemistry at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCR5 |
|---|---|
| Alternative Names | CCR5 Antibody: CKR5 CCR-5 CD195 CKR-5 CCCKR5 CMKBR5 IDDM22 CC-CKR-5 C-C chemokine receptor type 5 CHEMR13 C-C CKR-5 |
| Molecular Weight(MW) | Predicted: 39 kDa Observed: 40 kDa |
| Sequence Similarities | 3) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
Application
Immunohistochemistry of CCR5 in human lymph node tissue with CCR5 antibody at 20 μg/mL.
Application
Immunohistochemistry of CCR5 in human lymph node tissue with CCR5 antibody at 2.5 μg/ml.
Application
Western blot analysis of CCR5 in THP-1 whole cell lysate with CCR5 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1208 - THP-1 Cell Lysate |
|---|---|
| Application Notes | CCR5 antibody can be used for Western blot at 1 μg/mL. CCR5 antibody can also detect CCR5 by immunohistochemistry at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CCR5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CCR5 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM273846