Application
Immunohistochemical analysis of paraffin - embedded human normal lymph node showing cytoplasmic localization using CD19 antibody with DAB staining.Application
Western blot analysis using CD19 monoclonal antibody against truncated CD19 recombinant protein.| Product Name | CD19 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B-lymphocyte surface antigen B4B4, CVID3 |
| Product description | The CD19 antigen (95 kDa) is expressed from the earliest stage of B progenitor development, on all peripheral B cells including germinal centre B cells, and all B cell lines and B cell leukaemia tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturation to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.1) Rie, M.A. de, J. of Immunol. Methods, 1987. 102: 187. |
| Immunogen | Ni-NTA purified recombinant human CD19 expressed in E. Coli strain M15. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| Western Blot:Dilution 1:2000 - 5,000 IHC(P):Dilution 1:100 - 1:400 IHC(F):Dilution 1:200 - 1:400 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD19 |
|---|---|
| Alternative Names | B-lymphocyte surface antigen B4B4 CVID3 |
Application
Immunohistochemical analysis of paraffin - embedded human normal lymph node showing cytoplasmic localization using CD19 antibody with DAB staining.
Application
Western blot analysis using CD19 monoclonal antibody against truncated CD19 recombinant protein.| Application Notes | Western Blot:Dilution 1:2000 - 5,000 IHC(P):Dilution 1:100 - 1:400 IHC(F):Dilution 1:200 - 1:400 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CD19 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide. |
Data sheet for OM274161