Application
Western blot analysis of CD80 Antibody in HL-60 cell line lysates (35ug/lane)Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD80 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B71, B7, CD80, CD80, CD28LG, CD28LG1, LAB7 |
| Product description | CD80 is activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.1) Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010) |
| Immunogen | This CD80 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human CD80. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD80 |
|---|---|
| Alternative Names | T-lymphocyte activation antigen CD80 Activation B7-1 antigen BB1 CTLA-4 counter-receptor B71 B7 CD80 CD80 CD28LG CD28LG1 LAB7 |
| Molecular Weight(MW) | 33 kDa |
Application
Western blot analysis of CD80 Antibody in HL-60 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM274875