Application
Immunofluorescence of LAMP-1 inrat colon tissue with LAMP-1 antibody at 20 μg/ml.Application
Immunofluorescence of LAMP-1 in human colon tissue with LAMP-1 antibody at 20 μg/mL.Application
Western blot analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of LAMP-1 in rat colon tissue with LAMP-1 antibody at 2.5 μg/ml.| Product Name | LAMP-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAMP-1 Antibody: LAMPA, CD107a, LGP120, Lysosome-associated membrane glycoprotein 1, CD107 antigen-like family member A, LAMP-1 |
| Product description | LAMP-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome. LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression. However, the loss of both proteins resulted in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906. |
| Immunogen | LAMP-1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human LAMP-1. The immunogen is located within amino acids 230 - 280 of LAMP-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LAMP-1 antibody can be used for the detection of LAMP-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LAMP1 |
|---|---|
| Alternative Names | LAMP-1 Antibody: LAMPA CD107a LGP120 Lysosome-associated membrane glycoprotein 1 CD107 antigen-like family member A LAMP-1 |
| Molecular Weight(MW) | Predicted: 46 kDa Observed: 95 kDa |
| Sequence Similarities | 2) Cat. No. 1320 - Human Colon Tissue Lysate |
| Tissue Specificity | LAMP-1 antibody is predicted to not cross-react with LAMP-2. Due to extensive post-translational modification, LAMP-1 often migrates at higher than predicted molecular weight in SDS-PAGE. |
Application
Immunofluorescence of LAMP-1 inrat colon tissue with LAMP-1 antibody at 20 μg/ml.
Application
Immunofluorescence of LAMP-1 in human colon tissue with LAMP-1 antibody at 20 μg/mL.
Application
Western blot analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of LAMP-1 in rat colon tissue with LAMP-1 antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | LAMP-1 antibody can be used for the detection of LAMP-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LAMP-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LAMP-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM283221