Application
Immunocytochemistry of NAK in MOLT4 cells with NAK antibody at 10 μg/mL.| Product Name | NAK Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | NAK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. Phosphorylation of I-kappaB by IκB kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-κB. The IKK complex contains IKKα, IKKβ, and IKKγ. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-κB-activating kinase), and T2K. NAK/TBK1 activates IKKβ through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-κB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.1) Pomerantz JL, Baltimore D. NF-κB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J 1999;18(23):6694-704 |
| Immunogen | NAK antibody was raised against a synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human NAK/TBK1. The immunogen is located within the last 50 amino acids of NAK. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| NAK antibody can be used for detection of NAK/TBK1 by Western blot. An 84 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TBK1 |
|---|---|
| Alternative Names | NAK Antibody: NAK T2K NAK NF-kappa-B-activating kinase |
| Molecular Weight(MW) | 84 kDa |
| Sequence Similarities | 2) Cat. No. 17-006 - MOLT-4 Cell Slide |
| Tissue Specificity | No cross response to IKKa, IKKb, IKKy, or IKKe. |
Application
Immunocytochemistry of NAK in MOLT4 cells with NAK antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1206 - MOLT4 Cell Lysate |
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| Application Notes | NAK antibody can be used for detection of NAK/TBK1 by Western blot. An 84 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. |
| Form | Liquid |
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| Storage Instructions | NAK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NAK Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM285736