The NR1H4 gene encodes a protein that has a central role in lipid homeostasis and also regulates carbohydrate metabolism.1) Pineda et al., (2004) J. Biol. Chem. 279 (35), 36184-36191.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR1H4.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
NR1H4 antibody can be used for detection of NR1H4 by ELISA at 1:312500. NR1H4 antibody can be used for detection of NR1H4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on mouse glomerular endothelial at 1:1000.
Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human kidney.
Positive Control
1) Cat. No. XBL-10410 - Fetal Lung Tissue Lysate
Application Notes
NR1H4 antibody can be used for detection of NR1H4 by ELISA at 1:312500. NR1H4 antibody can be used for detection of NR1H4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR1H4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.