SMYD1 is a histone methyltransferases and plays a critical role in myofibril organization during myofiber maturation1) Phan, D., et al., (2005) Development 132 (11), 2669-2678.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse SMYD1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SMYD1 antibody can be used for detection of SMYD1 by ELISA at 1:1562500. SMYD1 antibody can be used for detection of SMYD1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Mouse NIH-3T3 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
SMYD1 antibody can be used for detection of SMYD1 by ELISA at 1:1562500. SMYD1 antibody can be used for detection of SMYD1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMYD1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.