The T-cell-specific transcription factor TCF7 activates genes involved in immune regulation and is a candidate locus for genetic susceptibility to type 1 diabetes.1) Smit, L., et al., (2004) J. Biol. Chem. 279 (17), 17232-17240.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF7.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
TCF7 antibody can be used for detection of TCF7 by ELISA at 1:62500. TCF7 antibody can be used for detection of TCF7 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
TCF7 antibody can be used for detection of TCF7 by ELISA at 1:62500. TCF7 antibody can be used for detection of TCF7 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TCF7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.