Application
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.| Product Name | Tfam Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | TFAM a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this protein is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns Sayre syndrome was produced when expression of the gene was eliminated by targeted disruption in heart and muscle cells.1) Noack, H., (2006) Biochim. Biophys. Acta 1760 (2), 141-150. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse Tfam. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Tfam antibody can be used for detection of Tfam by ELISA at 1:312500. Tfam antibody can be used for detection of Tfam by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Tfam |
|---|---|
| Alternative Names | Tfam AI661103 Hmgts mtTFA tsHMG |
| Molecular Weight(MW) | 27 kDa |
Application
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.| Positive Control | 1) SP2/0 Cell Lysate |
|---|---|
| Application Notes | Tfam antibody can be used for detection of Tfam by ELISA at 1:312500. Tfam antibody can be used for detection of Tfam by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store Tfam antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM293134