Application
Western blot analysis of YAP1 antibody in NCI-H460 cell line lysates (35ug/lane)Application
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | YAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, YAP1, YAP65 |
| Product description | YAP1 is the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.1) Cao,X., Genes Dev. 22 (23), 3320-3334 (2008) |
| Immunogen | This YAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-446 amino acids from the C-terminal region of human YAP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | YAP1 |
|---|---|
| Alternative Names | Transcriptional coactivator YAP1 Yes-associated protein 1 Protein yorkie homolog Yes-associated protein YAP65 homolog YAP1 YAP65 |
| Molecular Weight(MW) | 54 kDa |
Application
Western blot analysis of YAP1 antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM295282