IHC
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using lats1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using lats1 antibody with DAB staining.ICC/IF
Immunofluorescence analysis of C2C12 cells using lats1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Product Name | Anti-LATS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC |
| IHC:1:200-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LATS1 |
|---|---|
| Gene Synonyms | wts WARTS |
| Gene Full Name | large tumor suppressor kinase 1 |
| Gene Summary | The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017] |
| Molecular Weight(MW) | 127KD |
| Cellular Localization | Cytoplasm, Cytoskeleton |
IHC
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using lats1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using lats1 antibody with DAB staining.
ICC/IF
Immunofluorescence analysis of C2C12 cells using lats1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Application Notes | IHC:1:200-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM642559