WB
Western blot analysis of various lysates, using CD79a Rabbit mAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.IHC
Immunohistochemistry analysis of paraffinembedded Human breast using CD79a Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH6.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of Daudi and Jurkat(Negative sample） cells using CD79a Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | CD79a Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 165-225 of human CD79a (NP_001774.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD79A |
|---|---|
| Gene Synonyms | IGA MB1 MB-1 IGAlpha |
| Gene Full Name | CD79a molecule |
| Gene Summary | The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 25kDa(Observed MW 45-55kDa) |
| Cellular Localization | Cell membrane,Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates, using CD79a Rabbit mAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
IHC
Immunohistochemistry analysis of paraffinembedded Human breast using CD79a Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH6.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of Daudi and Jurkat(Negative sample） cells using CD79a Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:2000-1:10000 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
Data sheet for OM643105