WB
Various lysates were subjected to SDS PAGE followed by western blot with Alpha Tubulin antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Omnimabs® Plus 488 Alpha Tubulin antibody at dilution of 1:200.FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Omnimabs® Plus 488 Anti-Human Alpha Tubulin (red), or 0.4 ug Mouse IgG2b Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | Omnimabs® Plus 488-conjugated Alpha Tubulin Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human Tubulin-Alpha protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFWB |
| WB:1:300-1:600 ICC:1:50-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TUBA1B |
|---|---|
| Gene Synonyms | K-ALPHA-1 |
| Gene Full Name | tubulin alpha 1b |
| Gene Summary | Enables several functions, including GTP binding activity; GTPase activity; and ubiquitin protein ligase binding activity. A structural constituent of cytoskeleton. Involved in microtubule cytoskeleton organization. Located in microtubule. Is active in microtubule cytoskeleton. [provided by Alliance of Genome Resources, Jan 2025] |
| Molecular Weight(MW) | 50-55kDa |
| Cellular Localization | Cytoplasm, cytoskeleton. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Alpha Tubulin antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Omnimabs® Plus 488 Alpha Tubulin antibody at dilution of 1:200.
FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Omnimabs® Plus 488 Anti-Human Alpha Tubulin (red), or 0.4 ug Mouse IgG2b Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:300-1:600 ICC:1:50-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Data sheet for OM643837