Application
Rabbit Anti-EEA1 Antibody Catalog Number: Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver Observed Staining: Cytoplasm in hepatocytes, moderate signal, moderate tissue distribution Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 – 2.0 sec Protocol located in Reviews and Data.Application
WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/mlELISA Titer: 1:312500Positive Control: Human PlacentaApplication
Amount and Sample Type :500 ug rat brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :EEA1Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :EEA1Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science| Product Name | Rabbit anti-EEA1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MST105, ZFYVE2, MSTP105 |
| Immunogen | The immunogen for anti-EEA1 antibody: synthetic peptide directed towards the N terminal of human EEA1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EEA1 |
|---|---|
| Gene Synonyms | EEA1 |
| Gene Full Name | Early endosome antigen 1 |
| Gene Summary | EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25. |
| Alternative Names | MST105 ZFYVE2 MSTP105 |
| Molecular Weight(MW) | 162kDa |
| Sequence | 1411 amino acids |
Application
Rabbit Anti-EEA1 Antibody Catalog Number: Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver Observed Staining: Cytoplasm in hepatocytes, moderate signal, moderate tissue distribution Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 – 2.0 sec Protocol located in Reviews and Data.
Application
WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/mlELISA Titer: 1:312500Positive Control: Human Placenta
Application
Amount and Sample Type :500 ug rat brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :EEA1Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :EEA1Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM105120