Application
Immunohistochemistry with Human Uterus lysate tissueApplication
Immunohistochemistry with Human kidney lysate tissueApplication
WB Suggested Anti-STAT3 Antibody Titration: 1 ug/ml Positive Control: Human Liver| Product Name | Rabbit anti-STAT3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APRF, FLJ20882, MGC16063, HIES |
| Immunogen | The immunogen for anti-STAT3 antibody: synthetic peptide directed towards the N terminal of human STAT3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:2000 IHC:1:50~1:100 IF:1:100~1:500 |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT3 |
|---|---|
| Gene Synonyms | APRF FLJ20882 MGC16063 HIES |
| Gene Full Name | Signal transducer and activator of transcription 3 (acute-phase response factor) |
| Gene Summary | STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. |
| Alternative Names | APRF FLJ20882 MGC16063 HIES |
| Molecular Weight(MW) | 88kDa |
| Sequence | 769 amino acids |
Application
Immunohistochemistry with Human Uterus lysate tissue
Application
Immunohistochemistry with Human kidney lysate tissue
Application
WB Suggested Anti-STAT3 Antibody Titration: 1 ug/ml Positive Control: Human Liver| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:2000 IHC:1:50~1:100 IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM108644