WB
Western blot analysis using stat3 antibody against Raji(1), MCF-7(2), Jurkat(3), HepG2(4) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using stat3 antibody with DAB staining.ICC/IF
Immunofluorescence analysis of A549 cells using stat3 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Product Name | Anti-stat3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | STAT3 |
|---|---|
| Gene Synonyms | APRF HIES ADMIO ADMIO1 |
| Gene Full Name | signal transducer and activator of transcription 3 |
| Gene Summary | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. [provided by RefSeq, Aug 2020] |
| Molecular Weight(MW) | 88 kDa（Observed:68 kDa） |
| Source | Rabbit |
| Cellular Localization | Cytoplasm, Nucleus |
WB
Western blot analysis using stat3 antibody against Raji(1), MCF-7(2), Jurkat(3), HepG2(4) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using stat3 antibody with DAB staining.
ICC/IF
Immunofluorescence analysis of A549 cells using stat3 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Application Notes | WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM642126