Immunoblot
Lane 1: 7ug HeLa lysate+EGFP SiRNA Lane 2: 7ug HeLa lysate+UBE3A SiRNAPrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: UBE3ASubmitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto UniversityWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Product Name | Rabbit anti-UBE3A polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A |
| Immunogen | The immunogen for anti-UBE3A antibody: synthetic peptide directed towards the middle region of human UBE3A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE3A |
|---|---|
| Gene Synonyms | ANCR AS E6-AP EPVE6AP FLJ26981 HPVE6A |
| Gene Full Name | Ubiquitin protein ligase E3A |
| Gene Summary | UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. |
| Alternative Names | ANCR AS E6-AP EPVE6AP FLJ26981 HPVE6A |
| Molecular Weight(MW) | 101kDa |
| Sequence | 875 amino acids |
Immunoblot
Lane 1: 7ug HeLa lysate+EGFP SiRNA Lane 2: 7ug HeLa lysate+UBE3A SiRNAPrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: UBE3ASubmitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM111134