Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Mouse Brain| Product Name | Rabbit anti-Fto polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AW743446, mKIAA1752 |
| Immunogen | The immunogen for anti-Fto antibody: synthetic peptide corresponding to a region of Mouse |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHorseHumanMousePigRabbitRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Fto |
|---|---|
| Gene Synonyms | AW743446 mKIAA1752 |
| Gene Full Name | Fat mass and obesity associated |
| Gene Summary | Fto is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Fto has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Fto has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Fto has no activity towards 1-methylguanine and no detectable activity towards double-stranded DNA. Fto requires molecular oxygen, alpha-ketoglutarate and iron. Fto contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Fto contributes to the regulation of body size and body fat accumulation. |
| Alternative Names | AW743446 mKIAA1752 |
| Molecular Weight(MW) | 58kDa |
| Sequence | 502 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Mouse Brain| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM112878