| Product Name | FTO Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fat Mass and Obesity-associated Protein; FATSO |
| Product description | FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol. |
| Immunogen | Recombinant human FTO. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIPWB |
| ELISA: (direct and indirect: 1:2,000-1:5,000). Immunoprecipitation: (5ug/ml per sample in a final reaction volume of 500-1,000ul). Immunohistochemistry: (5-10ug/ml: paraffin sections). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | Fto |
|---|---|
| Alternative Names | Fat Mass and Obesity-associated Protein FATSO |
| Tissue Specificity | Recognizes human FTO. |
| Entrez Gene | 79068 |
|---|---|
| Protein Accession | NP_036066.2 |
| Application Notes | ELISA: (direct and indirect: 1:2,000-1:5,000). Immunoprecipitation: (5ug/ml per sample in a final reaction volume of 500-1,000ul). Immunohistochemistry: (5-10ug/ml: paraffin sections). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
Data sheet for OM279258