Immunofluorescence
Rat FRTL-5 cells Primary Antibody Dilution : 1:100 Secondary Antibody :Goat anti-rabbit-FITC Secondary Antibody Dilution : 1:1000Color/Signal Descriptions :1A: DAPI, 2B: phase contrast, 2A: CDH1, 2B: DAPI + CDH1Gene Name :CDH1Submitted by :Risheng Ma, MSSMWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Product Name | Rabbit anti-CDH1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Arc-1, CD324, CDHE, ECAD, LCAM, UVO |
| Immunogen | The immunogen for anti-CDH1 antibody: synthetic peptide directed towards the middle region of human CDH1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH1 |
|---|---|
| Gene Synonyms | Arc-1 CD324 CDHE ECAD LCAM UVO |
| Gene Full Name | Cadherin 1, type 1, E-cadherin (epithelial) |
| Gene Summary | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | Arc-1 CD324 CDHE ECAD LCAM UVO |
| Molecular Weight(MW) | 80kDa |
| Sequence | 882 amino acids |
Immunofluorescence
Rat FRTL-5 cells Primary Antibody Dilution : 1:100 Secondary Antibody :Goat anti-rabbit-FITC Secondary Antibody Dilution : 1:1000Color/Signal Descriptions :1A: DAPI, 2B: phase contrast, 2A: CDH1, 2B: DAPI + CDH1Gene Name :CDH1Submitted by :Risheng Ma, MSSM
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM114403