WB
Western blot analysis using CDH1 mouse mAb against PC-3 (1), A431 (2), MCF-7 (3), HT-29 (4), HePG2 (5), C6 (6), A549 (7), NIH/3T3 (8), and C2C12 (9) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using CDH1 mouse mAb with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using CDH1 mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using CDH1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)FC
Flow cytometric analysis of Hela cells using CDH1 mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to CDH1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human CDH1 (AA:extra(155-354)) expressed in E. Coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 FC:1:200-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH1 |
|---|---|
| Gene Synonyms | UVO CDHE ECAD LCAM Arc-1 BCDS1 CD324 |
| Gene Full Name | cadherin 1 |
| Gene Summary | This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015] |
| Molecular Weight(MW) | 97kDa |
| Cellular Localization | Cell membrane,Cytoplasm. |
WB
Western blot analysis using CDH1 mouse mAb against PC-3 (1), A431 (2), MCF-7 (3), HT-29 (4), HePG2 (5), C6 (6), A549 (7), NIH/3T3 (8), and C2C12 (9) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using CDH1 mouse mAb with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using CDH1 mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using CDH1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
FC
Flow cytometric analysis of Hela cells using CDH1 mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 FC:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM644032