Application
WB Suggested Anti-PCGF3 Antibody Titration: 0.125ug/mlELISA Titer: 1:12500Positive Control: Raji cell lysatePCGF3 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cellsApplication
Host: RabbitTarget Name: PCGF3Sample Type: Fetal Muscle lysatesAntibody Dilution: 1.0ug/mlApplication
Host: RabbitTarget Name: F13BSample Tissue: Human DLD1Antibody Dilution: 1.0ug/mlApplication
Rabbit Anti-F13B antibodyCatalog Number: ARP41999Formalin Fixed Paraffin Embedded Tissue: Human Fetal LiverPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0secApplication
Rabbit Anti-F13B antibodyCatalog Number: ARP41999Formalin Fixed Paraffin Embedded Tissue: Human VesselPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0secApplication
WB Suggested Anti-FBXO25 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human heartApplication
Host: RabbitTarget Name: MARCH5Sample Type: U937 Whole Cell lysatesAntibody Dilution: 1.0ug/mlApplication
WB Suggested Anti-SLC8A3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysateApplication
WB Suggested Anti-SULT6B1 Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysateApplication
WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/mlPositive Control: Human heartApplication
WB Suggested Anti-ZNF599 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysateApplication
WB Suggested Anti-SSX4B Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human kidneyApplication
WB Suggested Anti-UBQLN1 Antibody Titration: 1 ug/mlPositive Control: 293T cells lysateApplication
Immunohistochemistry with Human Lung, respiratory epethelium tissue at an antibody concentration of 5.0ug/ml using anti-UBQLN1 antibody (ARP54958_P050).Application
Anti-UBQLN1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. UBQLN1 Antibody ARP54958_P050 concentration 5 ug/ml.Application
WB Suggested Anti-Vwa5a AntibodyTitration: 1.0 ug/mlPositive Control: Mouse KidneyApplication
Host: RabbitTarget Name: SPATA2Sample Type: HCT15 Whole Cell lysatesAntibody Dilution: 1.0ug/mlApplication
WB Suggested Anti-ADAM8 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: PANC1 cell lysateApplication
WB Suggested Anti-ORMDL3 AntibodyTitration: 1.0 ug/mlPositive Control: Jurkat Whole CellApplication
Host: RabbitTarget Name: NR4A1Sample Tissue: Human HelaAntibody Dilution: 1.0ug/mlApplication
Rabbit Anti-NR4A1 AntibodyCatalog Number: ARP31941Paraffin Embedded Tissue: Human StomachCellular Data: Mucous CellsAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400XApplication
Rabbit Anti-NR4A1 AntibodyCatalog Number: ARP31941Paraffin Embedded Tissue: Human LiverCellular Data: HepatocytesAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400XApplication
Lanes: Lane 1: 50ug mouse embryonic fibroblast lysateLane 2: 50ug mouse PTRF-KO embryonic fibroblast lysateLane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF proteinPrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-Rabbit-HRPSecondary Antibody Dilution: 1:10000Gene Name: PTRFSubmitted by: Libin Liu, Boston University.Application
Host: RabbitTarget Name: PTRFSample Tissue: Mouse LungAntibody Dilution: 1.0ug/mlApplication
IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.Application
Lanes: Lane 1: 5 ug 293T cell lysateLane 2: 10 ug 293T cell lysateLane 3: 20 ug 293T cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10000Gene Name: HMGA2Submitted by: AnonymousHMGA2 is supported by BioGPS gene expression data to be expressed in HEK293TApplication
WB Suggested Anti-TRIM32 Antibody Titration: 1.25ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysateApplication
PKLR antibody - N-terminal region (ARP41699_T100) validated by WB using HepG2 cell lysate at 1.0ug/ml.There is BioGPS gene expression data showing that PKLR is expressed in HepG2Application
Rabbit Anti-PKLR AntibodyCatalog Number: ARP41699Paraffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400XApplication
Sample Type: Equine Cartilage ExplantsPrimary Dilution: 1:800Secondary Antibody: Bio-Rad 170-5046Secondary Dilution::100,000Image Submitted By: Adam WilliamsUniversity of NottinghamApplication
WB Suggested Anti-MMP1 Antibody Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate Primary Antibody Dilution : 1:500 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:2000 Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland BaltimoreApplication
Host: RabbitTarget Name: MMP1Sample Tissue: Human 293TAntibody Dilution: 1.0ug/mlApplication
Host: RabbitTarget Name: MARCH5Sample Type: Human Fetal KidneyAntibody Dilution: 1.0ug/mlApplication
Host: RabbitTarget Name: G6pcSample Tissue: Mouse LungAntibody Dilution: 1.0ug/mlApplication
Host: RabbitTarget Name: G6PCSample Tissue: Mouse TestisAntibody Dilution: 1ug/mlApplication
Host: RabbitTarget Name: SPPL2BSample Type: 293T Whole cell lysatesAntibody Dilution: 1.0ug/mlApplication
WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human SpleenApplication
WB Suggested Anti-NUP35 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysateApplication
WB Suggested Anti-Amigo2 AntibodyTitration: 1.0 ug/mlPositive Control: Rat HeartApplication
WB Suggested Anti-ACO2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysateACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cellsApplication
ACO2 antibody - N-terminal region (ARP56301_P050) validated by WB using Proximal kidney tubules purfied from cortex at 5.0ug/ml.Application
WB Suggested Anti-FFAR1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human LiverApplication
WB Suggested Anti-GLIS2 Antibody Titration: 2.5ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysateApplication
WB Suggested Anti-RBM14 Antibody Titration: 1.25ug/mlELISA Titer: 1:312500Positive Control: SP2/0 cell lysateApplication
Lanes: Lane 1: 50ug Hela LysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:10,000Gene Name: RBM14Submitted by: Archa Fox, University of Western Australia| Product Name | HIF1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide directed towards the N terminal of human HIF1A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| Species Reactivity | BovineHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIF1A |
|---|---|
| Gene Synonyms | PCGF3 |
| Gene Full Name | Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Gene Summary | Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). HIF1A is the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. |
| Alternative Names | RNF3 DONG1 RNF3A |
| Molecular Weight(MW) | 93kDa |
| Sequence | 826 amino acids |
Application
WB Suggested Anti-PCGF3 Antibody Titration: 0.125ug/mlELISA Titer: 1:12500Positive Control: Raji cell lysatePCGF3 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells
Application
Host: RabbitTarget Name: PCGF3Sample Type: Fetal Muscle lysatesAntibody Dilution: 1.0ug/ml
Application
Host: RabbitTarget Name: F13BSample Tissue: Human DLD1Antibody Dilution: 1.0ug/ml
Application
Rabbit Anti-F13B antibodyCatalog Number: ARP41999Formalin Fixed Paraffin Embedded Tissue: Human Fetal LiverPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec
Application
Rabbit Anti-F13B antibodyCatalog Number: ARP41999Formalin Fixed Paraffin Embedded Tissue: Human VesselPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec
Application
WB Suggested Anti-FBXO25 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human heart
Application
Host: RabbitTarget Name: MARCH5Sample Type: U937 Whole Cell lysatesAntibody Dilution: 1.0ug/ml
Application
WB Suggested Anti-SLC8A3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysate
Application
WB Suggested Anti-SULT6B1 Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate
Application
WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/mlPositive Control: Human heart
Application
WB Suggested Anti-ZNF599 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate
Application
WB Suggested Anti-SSX4B Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human kidney
Application
WB Suggested Anti-UBQLN1 Antibody Titration: 1 ug/mlPositive Control: 293T cells lysate
Application
Immunohistochemistry with Human Lung, respiratory epethelium tissue at an antibody concentration of 5.0ug/ml using anti-UBQLN1 antibody (ARP54958_P050).
Application
Anti-UBQLN1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. UBQLN1 Antibody ARP54958_P050 concentration 5 ug/ml.
Application
WB Suggested Anti-Vwa5a AntibodyTitration: 1.0 ug/mlPositive Control: Mouse Kidney
Application
Host: RabbitTarget Name: SPATA2Sample Type: HCT15 Whole Cell lysatesAntibody Dilution: 1.0ug/ml
Application
WB Suggested Anti-ADAM8 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: PANC1 cell lysate
Application
WB Suggested Anti-ORMDL3 AntibodyTitration: 1.0 ug/mlPositive Control: Jurkat Whole Cell
Application
Host: RabbitTarget Name: NR4A1Sample Tissue: Human HelaAntibody Dilution: 1.0ug/ml
Application
Rabbit Anti-NR4A1 AntibodyCatalog Number: ARP31941Paraffin Embedded Tissue: Human StomachCellular Data: Mucous CellsAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X
Application
Rabbit Anti-NR4A1 AntibodyCatalog Number: ARP31941Paraffin Embedded Tissue: Human LiverCellular Data: HepatocytesAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X
Application
Lanes: Lane 1: 50ug mouse embryonic fibroblast lysateLane 2: 50ug mouse PTRF-KO embryonic fibroblast lysateLane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF proteinPrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-Rabbit-HRPSecondary Antibody Dilution: 1:10000Gene Name: PTRFSubmitted by: Libin Liu, Boston University.
Application
Host: RabbitTarget Name: PTRFSample Tissue: Mouse LungAntibody Dilution: 1.0ug/ml
Application
IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.
Application
Lanes: Lane 1: 5 ug 293T cell lysateLane 2: 10 ug 293T cell lysateLane 3: 20 ug 293T cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10000Gene Name: HMGA2Submitted by: AnonymousHMGA2 is supported by BioGPS gene expression data to be expressed in HEK293T
Application
WB Suggested Anti-TRIM32 Antibody Titration: 1.25ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate
Application
PKLR antibody - N-terminal region (ARP41699_T100) validated by WB using HepG2 cell lysate at 1.0ug/ml.There is BioGPS gene expression data showing that PKLR is expressed in HepG2
Application
Rabbit Anti-PKLR AntibodyCatalog Number: ARP41699Paraffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X
Application
Sample Type: Equine Cartilage ExplantsPrimary Dilution: 1:800Secondary Antibody: Bio-Rad 170-5046Secondary Dilution::100,000Image Submitted By: Adam WilliamsUniversity of Nottingham
Application
WB Suggested Anti-MMP1 Antibody Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate Primary Antibody Dilution : 1:500 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:2000 Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland Baltimore
Application
Host: RabbitTarget Name: MMP1Sample Tissue: Human 293TAntibody Dilution: 1.0ug/ml
Application
Host: RabbitTarget Name: MARCH5Sample Type: Human Fetal KidneyAntibody Dilution: 1.0ug/ml
Application
Host: RabbitTarget Name: G6pcSample Tissue: Mouse LungAntibody Dilution: 1.0ug/ml
Application
Host: RabbitTarget Name: G6PCSample Tissue: Mouse TestisAntibody Dilution: 1ug/ml
Application
Host: RabbitTarget Name: SPPL2BSample Type: 293T Whole cell lysatesAntibody Dilution: 1.0ug/ml
Application
WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Spleen
Application
WB Suggested Anti-NUP35 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate
Application
WB Suggested Anti-Amigo2 AntibodyTitration: 1.0 ug/mlPositive Control: Rat Heart
Application
WB Suggested Anti-ACO2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysateACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Application
ACO2 antibody - N-terminal region (ARP56301_P050) validated by WB using Proximal kidney tubules purfied from cortex at 5.0ug/ml.
Application
WB Suggested Anti-FFAR1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human Liver
Application
WB Suggested Anti-GLIS2 Antibody Titration: 2.5ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate
Application
WB Suggested Anti-RBM14 Antibody Titration: 1.25ug/mlELISA Titer: 1:312500Positive Control: SP2/0 cell lysate
Application
Lanes: Lane 1: 50ug Hela LysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:10,000Gene Name: RBM14Submitted by: Archa Fox, University of Western Australia| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM122038