Immunoblot
Lane 1: 80ug Chicken liver (nuclei)Primary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-rabbit IgGSecondary Antibody Dilution: 1:2,000Gene Name: HIF1ASubmitted by: Silvia Moore, Weill Medical College of Cornell UniversityWestern blot
HIF1A antibody - middle region validated by WB using CoCl2 at 1:500.Western blot
HIF1A antibody - middle region validated by WB using chicken liveWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Small Intestine| Product Name | Rabbit anti-HIF1A polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78 |
| Immunogen | The immunogen for anti-HIF1A antibody: synthetic peptide directed towards the middle region of human HIF1A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIF1A |
|---|---|
| Gene Synonyms | HIF-1alpha HIF1 HIF1-ALPHA MOP1 PASD8 bHLHe78 |
| Gene Full Name | Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Gene Summary | Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha s |
| Alternative Names | HIF-1alpha HIF1 HIF1-ALPHA MOP1 PASD8 bHLHe78 |
| Molecular Weight(MW) | 93kDa |
| Sequence | 826 amino acids |
Immunoblot
Lane 1: 80ug Chicken liver (nuclei)Primary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-rabbit IgGSecondary Antibody Dilution: 1:2,000Gene Name: HIF1ASubmitted by: Silvia Moore, Weill Medical College of Cornell University
Western blot
HIF1A antibody - middle region validated by WB using CoCl2 at 1:500.
Western blot
HIF1A antibody - middle region validated by WB using chicken live
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Small Intestine| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM108551