Application
A. Western blot analysis on Molt-4 cell lysate using anti-TRAF3 RabMAb (Cat. #AJ1787a), dilution 1:500.Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-TRAF3 RabMAb (Cat. #AJ1787a).| Product Name | TRAF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF3, CAP1, CRAF1, TNF receptor-associated factor 3, LMP1-associated protein;LAP1;CAP-1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1730Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAP1 CRAF1 |
|---|---|
| Alternative Names | TRAF3 CAP1 CRAF1 TNF receptor-associated factor 3 LMP1-associated protein LAP1 CAP-1 |
| Molecular Weight(MW) | 64490 Da |
| Function | Regulates pathways leading to the activation of NF- kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa- B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14 |
| Tissue Specificity | A synthetic peptide corresponding to residues on the N-terminus of human TRAF3 was used as an immunogen. |
| Cellular Localization | Cytoplasm (Probable). Endosome (By similarity). Mitochondrion. Note=Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus |
| Entrez Gene | 7187 |
|---|
Application
A. Western blot analysis on Molt-4 cell lysate using anti-TRAF3 RabMAb (Cat. #AJ1787a), dilution 1:500.
Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-TRAF3 RabMAb (Cat. #AJ1787a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM223504