Application
Western blot analysis of HMGA1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). HMGA1 (arrow) was detected using the purified Pab.Application
Western blot analysis of HMGA1 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). HMGA1 (arrow) was detected using the purified Pab.Application
HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of HMGA1 (arrow) using rabbit polyclonal HMGA1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGA1 gene.| Product Name | HMGA1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HMGIY |
|---|---|
| Alternative Names | HMGA1 HMGIY High mobility group protein HMG-I/HMG-Y High mobility group AT-hook protein 1 High mobility group protein R |
| Molecular Weight(MW) | 11676 Da |
| Function | HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions |
| Tissue Specificity | This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71~101 amino acids from the C-terminal region of human HMGA1. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3159 |
|---|
Application
Western blot analysis of HMGA1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). HMGA1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of HMGA1 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). HMGA1 (arrow) was detected using the purified Pab.
Application
HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of HMGA1 (arrow) using rabbit polyclonal HMGA1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMGA1 gene.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Data sheet for OM235163