Application
Western blot analysis of ADCY8 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ADCY8 (arrow) was detected using the purified Pab.Application
ADCY8 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded mouse brain tissue reacted with ADCY8 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ADCY8 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ADCY8 (Internal)Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. The enzymatic activity is under the control of several hormones, and different polypeptides participate in the transduction of the signal from the receptor to the catalytic moiety. Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 952~982 amino acids from the Center region of human ADCY8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADCY8 Adenylyl cyclase 8 ATP pyrophosphate-lyase 8 Ca(2+)/calmodulinactivated adenylyl cyclase Adenylate cyclase type8 Ca(2+)/calmodulin-activated adenylyl cyclase Adenylate cyclase type VIII Adenylyl cyclase8 ATP pyrophosphatelyase 8 ATP pyrophos |
|---|---|
| Target | ADCY8 |
| Molecular Weight(MW) | 140.122 kDa (note) |
Application
Western blot analysis of ADCY8 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ADCY8 (arrow) was detected using the purified Pab.
Application
ADCY8 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded mouse brain tissue reacted with ADCY8 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
Data sheet for OM238299