Application
Formalin-fixed and paraffin-embedded mouse brain tissue reacted with ADCY8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.| Product Name | ADCY8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenylate cyclase type 8, ATP pyrophosphate-lyase 8, Adenylate cyclase type VIII, Adenylyl cyclase 8, AC8, Ca(2+)/calmodulin-activated adenylyl cyclase, ADCY8 |
| Product description | ADCY8 is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. The enzymatic activity is under the control of several hormones, and different polypeptides participate in the transduction of the signal from the receptor to the catalytic moiety. Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase provided by RefSeq].1) Martin,A.C.,et.al., Mol. Pharmacol. 75 (4), 830-842 (2009) |
| Immunogen | This ADCY8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 946-972 amino acids from the Central region of human ADCY8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADCY8 |
|---|---|
| Alternative Names | Adenylate cyclase type 8 ATP pyrophosphate-lyase 8 Adenylate cyclase type VIII Adenylyl cyclase 8 AC8 Ca(2+)/calmodulin-activated adenylyl cyclase ADCY8 |
| Molecular Weight(MW) | 140 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded mouse brain tissue reacted with ADCY8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM270139