Application
WB analysis of Jurkat cells using CENPB antibody at 0.2-1μg/ml.| Product Name | CENPB antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CENPBThis gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to a C-terminal region of Human CENPB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Cow: 83%; Horse: 86%; Human: 100%; Mouse: 93%; Pig: 86%; Rat: 100%; Sheep: 86%; Yeast: 100%; Zebrafish: 75% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CENPB |
|---|---|
| Target | CENPB |
| Molecular Weight(MW) | 65 KDa (note) |
Application
WB analysis of Jurkat cells using CENPB antibody at 0.2-1μg/ml.| Positive Control | Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Data sheet for OM246653