| Product Name | Collagen VII antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LH7.2] to Collagen VIIThis gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq] |
| Immunogen | Full length native protein (purified) (Human). Insoluble fractions prepared from neonatal foreskin epidermal cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | LH7.2 |
| Host Species | Mouse |
| Tested Applications | DotELISAICC/IFIHC-FWB |
| Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/1000. (human or other mammalian frozen sections). IHC-Fr: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanPigGoatGuinea PigHorseSheepBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EBR1 Collagen VII 120120 1294 Q02388 EBD1 COL7A1 EBDCT |
|---|---|
| Target | Collagen VII |
| Tissue Specificity | Reactive in immunoblotting against intact tissue Type VII collagen (250kD). Pepsin digestion of terminal regions leads to complte loss of reactivity. Antibody reactivity is conserved after treatment with collagenase.No reactivity against collagen types I, II, III, IV, V, laminin and fibronectin.The antibody reacts predominantly with the basement membrane zone of stratified ssquamous epithelia and ectodermally derived glands. |
| Application Notes | Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/1000. (human or other mammalian frozen sections). IHC-Fr: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascites fluid containing 0.1% Sodium Azide |
Data sheet for OM247807