Application
Antibody is used in Western blot to detect ABCB11 in mouse liver tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | ABCB11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bile salt export pump, ATP-binding cassette sub-family B member 11, ABCB11, BSEP |
| Product description | ABCB11 is involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. It is expressed predominatly, if not exclusively, in the liver, where it is further localized to the canilicular microvilli and to subcanilicular vesicles fo the hepatocytes. Structurally, ABCB11 is a multifunctional polypeptide with two homologus halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.1) Chen, H.L., et al., J. Pediatr. 140(1):119-124 (2002). Saito, S., et al., J. Hum. Genet. 47(1):38-50 (2002). Strautnieks, S.S., et al., Nat. Genet. 20(3):233-238 (1998). |
| Immunogen | This ABCB11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1038-1067 amino acids from the C-terminal region of human ABCB11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | ABCB11 |
|---|---|
| Alternative Names | Bile salt export pump ATP-binding cassette sub-family B member 11 ABCB11 BSEP |
| Molecular Weight(MW) | 146 kDa |
Application
Antibody is used in Western blot to detect ABCB11 in mouse liver tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM269568