Application
Western blot analysis of lysate from HepG2 cell line, using ADRB2 Antibody (S364) at 1:1000.Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of anti-ADRB2 Antibody (S364) in NCI-H460 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain reacted with ADRB2 Antibody (S364), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of NCI-H292 cells using ADRB2 Antibody (S364)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ADRB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, Beta-2 adrenoceptor, ADRB2, ADRB2R, B2AR |
| Product description | ADRB2, beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This protein is intronless.1) Kobilka B.K., Dixon R.A.F.Proc. Natl. Acad. Sci. U.S.A. 84:46-50(1987) |
| Immunogen | This ADRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-373 amino acids from human ADRB2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADRB2 |
|---|---|
| Alternative Names | Beta-2 adrenergic receptor Beta-2 adrenoreceptor Beta-2 adrenoceptor ADRB2 ADRB2R B2AR |
| Molecular Weight(MW) | 46 kDa |
Application
Western blot analysis of lysate from HepG2 cell line, using ADRB2 Antibody (S364) at 1:1000.
Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of anti-ADRB2 Antibody (S364) in NCI-H460 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain reacted with ADRB2 Antibody (S364), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of NCI-H292 cells using ADRB2 Antibody (S364)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM270012