Application
Immunohistochemical analysis of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody at 1:1000.Application
Western blot analysis of lysate from human placenta tissue,using ALPI Antibody at 1:1000 at each laneApplication
Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Alkaline Phosphatase (ALPI) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1| Product Name | Alkaline Phosphatase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI |
| Product description | There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.1) References for protein: |
| Immunogen | This Alkaline Phosphatase (ALPI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human Alkaline Phosphatase (ALPI). |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:100 For IF starting dilution is: 1:100: |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALPI |
|---|---|
| Alternative Names | Intestinal-type alkaline phosphatase IAP Intestinal alkaline phosphatase ALPI |
| Molecular Weight(MW) | 57 kDa |
Application
Immunohistochemical analysis of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody at 1:1000.
Application
Western blot analysis of lysate from human placenta tissue,using ALPI Antibody at 1:1000 at each lane
Application
Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Alkaline Phosphatase (ALPI) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:100 For IF starting dilution is: 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM270890