CaM Kinase II (also known as CAMK2 and calcium/calmodulin-dependent protein kinase type II alpha chain) is a prominent kinase in the central nervous system that may function in the long-term potentiation of neurotransmitter release. CaM Kinase II autophosphorylates itself at Thr-286 which allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. CaM Kinase II is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. This kinase is expressed in brain tissue. Alternative splicing occurs for this gene product.
Immunogen
CAM Kinase 2 alpha antibody was raised against amino acids 6-23 of CAM Kinase 2 alpha (Human).
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIFIHCWB
CAM Kinase 2 alpha antibody can be used in Western Blot starting at 5 - 10 μg/mL, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation.:
Immunohistochemistry staining of CAM Kinase 2 alpha in brain cortex tissue using CAM Kinase 2 alpha Antibody.
Application Notes
CAM Kinase 2 alpha antibody can be used in Western Blot starting at 5 - 10 μg/mL, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation.:
Additional Information
Form
Liquid
Storage Instructions
Store CAM Kinase 2 alpha antibody at -20 ˚C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 ˚C or below. As with all antibodies avoid freeze/thaw cycles.
Storage Buffer
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.