Application
Western blot analysis in A549,K562 cell line lysates (35ug/lane).Application
Western Blot at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Antibody staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | CD11b Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A |
| Product description | CD11b is the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.1) Gjelstrup, L.C., et al. J. Immunol. 185(7):4154-4168(2010) |
| Immunogen | This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:500 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITGAM |
|---|---|
| Alternative Names | Integrin alpha-M CD11 antigen-like family member B CR-3 alpha chain Cell surface glycoprotein MAC-1 subunit alpha Leukocyte adhesion receptor MO1 Neutrophil adherence receptor CD11b ITGAM CD11B CR3A |
| Molecular Weight(MW) | 127 kDa |
Application
Western blot analysis in A549,K562 cell line lysates (35ug/lane).
Application
Western Blot at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.
Application
Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Antibody staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:500 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM273930