Application
Western blot analysis of DIO1 Antibody in HepG2 cell line lysates (35ug/lane)Application
Western Blot at 1:2000 dilution + human kidney whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | DIO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Type I iodothyronine deiodinase, 5DI, DIOI, Type 1 DI, Type-I 5'-deiodinase, DIO1, ITDI1, TXDI1 |
| Product description | DIO1 is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD).1) Landa,I., et.al., PLoS Genet.(9), E1000637 (2009) |
| Immunogen | This DIO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-57 amino acids from the N-terminal region of human DIO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIO1 |
|---|---|
| Alternative Names | Type I iodothyronine deiodinase 5DI DIOI Type 1 DI Type-I 5'-deiodinase DIO1 ITDI1 TXDI1 |
| Molecular Weight(MW) | 29 kDa |
Application
Western blot analysis of DIO1 Antibody in HepG2 cell line lysates (35ug/lane)
Application
Western Blot at 1:2000 dilution + human kidney whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM277039