Application
Western blot analysis of LRP6 using rabbit polyclonal LRP6 Antibody (C-term T1546) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LRP6 gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with LRP6 Antibody (C-term T1546), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | LRP6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6 |
| Product description | LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.1) Liu, G., et al., Mol. Cell. Biol. 23(16):5825-5835 (2003). Tamai, K., et al., Nature 407(6803):530-535 (2000). Brown, S.D., et al., Biochem. Biophys. Res. Commun. 248(3):879-888 (1998). |
| Immunogen | This LRP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1531-1560 amino acids from the C-terminal region of human LRP6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | LRP6 |
|---|---|
| Alternative Names | Low-density lipoprotein receptor-related protein 6 LRP-6 LRP6 |
| Molecular Weight(MW) | 180 kDa |
Application
Western blot analysis of LRP6 using rabbit polyclonal LRP6 Antibody (C-term T1546) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LRP6 gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with LRP6 Antibody (C-term T1546), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM283742