Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells treated with UV using p90 RSK (Phospho-Thr573) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | p90 RSK (phospho Thr573) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KS6A1, p90RSK1, RSK1, RPS6KA1 |
| Product description | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes.1) Eiichi Takahashi, J. Biol. Chem., Jul 1999; 274: 20206. |
| Immunogen | p90 RSK (phospho Thr573) antibody was raised against a peptide sequence around phosphorylation site of threonine 573 (L-M-T (p)-P-C) derived from Human p90 RSK. |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KA1 |
|---|---|
| Alternative Names | KS6A1 p90RSK1 RSK1 RPS6KA1 |
| Molecular Weight(MW) | 95 kDa |
| Tissue Specificity | The antibody detects endogenous levels of P90RSK only when phosphorylated at threonine 573. |
| Entrez Gene | 6195 |
|---|---|
| Protein Accession | NP_002944.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells treated with UV using p90 RSK (Phospho-Thr573) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM287219