Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using p90RSK (Phospho-Thr348).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90RSK (Phospho-Thr348). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p90RSK (Phospho-Thr348).| Product Name | p90RSK (phospho Thr348) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RSK, HU-1, RSK1, MAPKAPK1A, MEK3, MKK3, PRKMK3, SKK2, KS6A1, MAPKAP-K1a, RPS6KA1, S6K-alpha 1 |
| Product description | Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.1) Silverman E, et al. Mol Cell Biol. 2004 Dec; 24 (24): 10573-10583. Andrew D, et al. Biochem J. 2006 February 1; 393 (Pt 3): 715–724. Itoh S, et al. J Biol Chem 2005 Jun 24; 280 (25): 24135-24142 |
| Immunogen | p90RSK (Phospho-Thr348) antibody was raised against a peptide sequence around phosphorylation site of threonine 348 (S-R-T (p) -P-R) derived from Human p90RSK. |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KA1 |
|---|---|
| Alternative Names | RSK HU-1 RSK1 MAPKAPK1A MEK3 MKK3 PRKMK3 SKK2 KS6A1 MAPKAP-K1a RPS6KA1 S6K-alpha 1 |
| Molecular Weight(MW) | 90 kDa |
| Tissue Specificity | This antibody detects endogenous level of p90RSK only when phosphorylated at threonine 348. |
| Entrez Gene | 6195 |
|---|---|
| Protein Accession | NP_001006666.1 |
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using p90RSK (Phospho-Thr348).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90RSK (Phospho-Thr348). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p90RSK (Phospho-Thr348).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM287223