Application
Immunofluorescence of SHP2 in Human Skeletal Muscle cells with SHP2 antibody at 20 μg/mL.Application
Western blot analysis of SHP2 in mouse skeletal muscle tissue lysate with SHP2 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SHP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHP2 Antibody: Src homology-2 domain containing protein (SHP2) is a member of the protein tyrosine phosphatase (PTP) family, a protein family that contains signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. SHP2 contains two tandem Src homology-2 (SH2) domains, which function as phosphotyrosine binding domains either directly or through scaffolding intermediates such as the insulin-receptor substrate 1 (IRS-1). These SH2 domains mediate the interaction of SHP2 with its substrates, allowing SHP2 to dephosphorylate proteins that inhibit signaling kinases such as ERK1 and AKT. SHP2 is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Recent experiments have shown SHP2 plays a significant role in hepatoprotection and liver regeneration.1) Yu Z, Ahmad S, Schwartz JL, et al. Protein-tyrosine phosphatase SHP2 is positively linked to proteinase-activated receptor 2-mediated mitogenic pathway. J. Biol. Chem.1997; 272:7519-24. |
| Immunogen | SHP2 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human SHP2. The immunogen is located within the first 50 amino acids of SHP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| SHP2 antibody can be used for the detection of SHP2 by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTPN11 |
|---|---|
| Alternative Names | SHP2 Antibody: CFC NS1 SHP2 BPTP3 PTP2C PTP-1D SH-PTP2 SH-PTP3 SHPTP2 Tyrosine-protein phosphatase non-receptor type 11 Protein-tyrosine phosphatase 1D |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (100%) |
Application
Immunofluorescence of SHP2 in Human Skeletal Muscle cells with SHP2 antibody at 20 μg/mL.
Application
Western blot analysis of SHP2 in mouse skeletal muscle tissue lysate with SHP2 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | SHP2 antibody can be used for the detection of SHP2 by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
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| Storage Instructions | SHP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SHP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM291158