Application
Western blot analysis of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 μg/mL.Application
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 μg/mL.| Product Name | TRAF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF3 Antibody: CAP1, LAP1, CAP-1, CRAF1, IIAE5, CD40bp, CAP1, TNF receptor-associated factor 3 |
| Product description | TRAF3 Antibody: Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1. Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-κB. Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTbetaR.1) Arch RH, Gedrich RW, and Thompson CB. Tumor necrosis factor receptor-associated factors (TRAFs) - a family of adaptor proteins that regulate life and death.Genes Dev. 1998; 12:2821-30. |
| Immunogen | TRAF3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human. The immunogen is located within amino acids 20 - 70 of TRAF3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| TRAF3 antibody can be used for the detection of TRAF3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRAF3 |
|---|---|
| Alternative Names | TRAF3 Antibody: CAP1 LAP1 CAP-1 CRAF1 IIAE5 CD40bp CAP1 TNF receptor-associated factor 3 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Western blot analysis of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 μg/mL.
Application
Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | TRAF3 antibody can be used for the detection of TRAF3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRAF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRAF3 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM293877