WB
Western blot analysis using CD142 antibody against Raji (1), HepG2 (2), Hela (3), HUVEC (4) cell lysate.ICC/IF
Immunofluorescence analysis of Hela cells using CD142 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Product Name | Anti-CD142 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB:1:200-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | F3 |
|---|---|
| Gene Synonyms | TF TFA CD142 F3 tissue factor |
| Gene Full Name | coagulation factor III, tissue factor |
| Gene Summary | This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Aug 2020] |
| Molecular Weight(MW) | 33 kDa（Observed:50 kDa） |
| Source | Rabbit |
| Cellular Localization | Plasma Membrane,Extracellular region or secreted |
WB
Western blot analysis using CD142 antibody against Raji (1), HepG2 (2), Hela (3), HUVEC (4) cell lysate.
ICC/IF
Immunofluorescence analysis of Hela cells using CD142 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Application Notes | WB:1:200-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM642039