WB
Western blot analysis of various lysates, using Coagulation factor III/Tissue Factor Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG(H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffin embedded Human lung adenocarcinoma tissue using Coagulation factor III/Tissue Factor Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of A-431 and MCF7(Negative sample) cells using Coagulation factor III/Tissue Factor Rabbit mAb at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Coagulation factor III/Tissue Factor Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 34-251 of human Coagulation factor III/Tissue Factor. (NP_001984.1). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:100-1:500 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | F3 |
|---|---|
| Gene Synonyms | TF TFA CD142 |
| Gene Full Name | coagulation factor III, tissue factor |
| Gene Summary | This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Aug 2020] |
| Molecular Weight(MW) | 45-53kDa |
| Cellular Localization | Membrane, Secreted, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates, using Coagulation factor III/Tissue Factor Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG(H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffin embedded Human lung adenocarcinoma tissue using Coagulation factor III/Tissue Factor Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of A-431 and MCF7(Negative sample) cells using Coagulation factor III/Tissue Factor Rabbit mAb at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Data sheet for OM643886