WB
Western blot analysis using CDCA8 antibody against PANC-1(1), MCF-7(2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CDCA8 antibody with DAB staining.ICC/IF
Immunofluorescence analysis of MG-63 cells using CDCA8 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Product Name | Anti- CDCA8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDCA8 |
|---|---|
| Gene Synonyms | BOR DasraB MESRGP BOREALIN |
| Gene Full Name | cell division cycle associated 8 |
| Gene Summary | This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. |
| Molecular Weight(MW) | 31 kDa |
| Cellular Localization | Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus |
WB
Western blot analysis using CDCA8 antibody against PANC-1(1), MCF-7(2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CDCA8 antibody with DAB staining.
ICC/IF
Immunofluorescence analysis of MG-63 cells using CDCA8 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Application Notes | WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM642407