WB
Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit pAb at 1:1000 dilution. Secondary antibody:HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.IHC
Immunohistochemistry analysis of paraffin embedded Rat brain using Parvalbumin (PVALB) Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IF-P
Immunofluorescence analysis of Human kidney tissue using Parvalbumin/PVALB Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.| Product Name | Parvalbumin/PVALB Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human Parvalbumin (PVALB) (NP_002845.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PVALB |
|---|---|
| Gene Synonyms | D22S749 |
| Molecular Weight(MW) | 12kDa(Observed MW 15kDa) |
| Cellular Localization | axon,cytoplasm,nucleus,synapse. |
WB
Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit pAb at 1:1000 dilution. Secondary antibody:HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
IHC
Immunohistochemistry analysis of paraffin embedded Rat brain using Parvalbumin (PVALB) Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IF-P
Immunofluorescence analysis of Human kidney tissue using Parvalbumin/PVALB Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
Data sheet for OM643321