WB
Western blot analysis of various lysates, using Human Kappa Light Chain Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): HAP1 Exposure time: 60s.IHC
Immunohistochemistry analysis of Human Kappa Light Chain in paraffin-embedded human tonsil using Human Kappa Light Chain Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.IF-P
Immunofluorescence analysis of paraffin embedded human tonsil using Human Kappa Light Chain Rabbit mAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Human Kappa Light Chain Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-107 of human Kappa Light Chain(P01834). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:1000 IHC:1:500-1:1000 IF-P:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IGKC |
|---|---|
| Gene Synonyms | Km HCAK1 IGKCD |
| Gene Full Name | immunoglobulin kappa constant |
| Gene Summary | Predicted to enable antigen binding activity. Involved in adaptive immune response. Located in blood microparticle and extracellular exosome. Part of IgG immunoglobulin complex. [provided by Alliance of Genome Resources, Dec 2024] |
| Molecular Weight(MW) | 12kDa(Observed MW 25kDa) |
| Cellular Localization | Cell membrane , Secreted. |
WB
Western blot analysis of various lysates, using Human Kappa Light Chain Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): HAP1 Exposure time: 60s.
IHC
Immunohistochemistry analysis of Human Kappa Light Chain in paraffin-embedded human tonsil using Human Kappa Light Chain Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
IF-P
Immunofluorescence analysis of paraffin embedded human tonsil using Human Kappa Light Chain Rabbit mAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IHC:1:500-1:1000 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
Data sheet for OM643515